Chloride is important for fish, nitrates have a similar structured chloride and can be absorbed instead. To reduce this, sodium chloride (aquarium salt)
Nitrates can oxidize the iron in hemoglobin from its ferrous (Fe²⁺) state (which binds oxygen) to the ferric (Fe³⁺) state, forming methemoglobin.
Methemoglobin cannot bind oxygen effectively, reducing the blood’s ability to transport oxygen to tissues.
•ion regulation disruption
Fish “osmoregulate” by moving ions like sodium, potassium, and chloride across their bodies cells.
Nitrate (NO₃⁻) ions have a similar structure to chloride ions, meaning they can compete for absorption in the gills, this disrupts osmoregulation.
•Overhydration
In general freshwater fish regulate water through their bodies. When the aquarium water is absorbed through the body your fish excrete it through urine.
Prolonged exposure to nitrates weaken their immune system, this can include the liver. The liver is responsible for passing water through to urine.
This can lead to the body pulling in the same amount of water but not being able to push it out fast enough, this can cause swelling or even explosion of the cells.